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Mouse Development, Stem Cells & Cancer

Cold Spring Harbor, NY

June 5–March 24, 2019

This intensive lecture and laboratory course is designed for scientists interested in using mouse models to study mammalian development, stem cells and cancer. The lecture portion of the course, taught by leaders in the field, provides the conceptual basis for contemporary research in embryogenesis, organogenesis in development and disease, embryonic, adult and induced pluripotent stem cells and cancer biology.

The laboratory and workshop portions of the course provide hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses.

Experimental techniques include:
  • Genome editing by CRISPR/Cas9
  • Zygote microinjection
  • Isolation and culture/manipulation of pre- and post-implantation embryos
  • Embryo transfer
  • Embryo electroporation
  • Roller bottle culture
  • Sperm cryopreservation
  • in vitro fertilization
  • Culture of mouse embryonic stem cells and fibroblasts
  • Synthetic embryo generation
  • Vibratome and cryosectioning
  • in situ RNA hybridization
  • Immunostaining
  • FACS sorting
  • Analysis of hematopoietic stem cells, skeletal preparation, organ explant culture and fluorescent imaging, including live time-lapse microscopy.